Validation of the comparative quantification method of real-time PCR analysis and a cautionary tale of housekeeping gene selection

Richard D. McCurdy*, John J. McGrath, Alan Mackay-Sim Eskitis Institute for Cell and Molecular Therapies, and School of Biomedical and Biomolecular Science, Griffith University, Brisbane, QLD 4111, Australia Queensland Centre for Mental Health Research, The Park Centre for Mental Health, Wacol QLD 4076, Australia Department of Psychiatry, University of Queensland, St Lucia QLD 4072, Australia __________________________________________________________________________________ *Correspondence: Dr. Richard D. McCurdy, Sick Kids Research Institute, Program in Genetics and Genomic Biology, TMDT Building, East Tower, 15th Floor, 101 College St., Toronto, Ontario CANADA M5G 1L7; Tel: 1-416-813-7654 X 8164; Fax: 1-416813-4931; e-mail: [email protected] #Present Address: Sick Kids Research Institute, Genetics and Genomic Biology, TMDT Building, 101 College St., 15 Floor, East Tower, Toronto, ON M5G 1L7, Canada